DR. MARTA CLOS-SANSALVADOR. (Institut d’Investigació en Ciències de la Salut Germans Trias i Pujol (IGTP). Badalona, Spain).

10:00-10:30 Isolation of Extracellular Vesicles. DR. FRANCESC E. BORRÀS & MARTA SANROQUE-MUÑÓZ. (Institut d’Investigació en Ciències de la Salut Germans Trias i Pujol (IGTP). Badalona, Spain).

10:30-11:00 EV uptake and functional assays. DR. MARÍA YAÑEZ-MO & MIGUEL PALMA-COBO.

11:30-11:45 EV cargo preparation for OMICS analysis. DR. ROCIO BRAVO-MIANA. (IIS Biogipuzkoa, San Sebastian, Spain).

11:45-12:00 Single-vesicle phenotyping. DR. ANA TALAMILLO. (CIC bioGUNE-BRTA. Bilbao, Spain).

12:00-12:30 Extracellular Vesicles in Therapy. DR. CLARA I. RODRÍGUEZ & LEIRE CABODEVILLA. (Biobizkaia Health Research Institute, Bilbao, Spain).

12:30-13:00 Extracellular Vesicles as Diagnostic Tool. DR. DAVID OTAEGI & JONE ARIZAGA. (IIS Biogipuzkoa, San Sebastian, Spain).

14:00 – 14:48  Demonstration HEKAT

14:48 – 15:36  Demonstration BD

15:36 – 16:24  Demonstration BIO-CONNECT

16:24 – 17:12  Demonstration HANSA BIOMED

17:12 – 18:00  Demonstration BGI-TECH

MARCEL-LA FRANQUESA BARTOLOME. (Germans Trias i Pujol Research Institute (IGTP), Badalona, Spain. President of GEIVEX). JUANMA FALCÓN-PÉREZ (CIC bioGUNE-BRTA, CIBERehd, IKERBASQUE Research Foundation, Bilbao, Spain. Chair of Local Organizing Committee). JON UMEREZ URREZOLA (Vicerrector de Investigación (EHU)). AMAIA ESQUISABEL ALEGRÍA

CHAIR: DR. AINTZANE APRAIZ (University of the Basque Country (EHU), Bilbao, Spain).

9:00 Keynote Lecture “Navigating Complexity in Extracellular Vesicle Research”. PROF. EDIT BUZAS (Department of Genetics, Cell and Immunobiology at Semmelweis University, Budapest, Hungary).

9:45 Selected talk: “CHARACTERIZATION OF SECRETED CEMIP, A MELANOMA-DERIVED EV CORONA PROTEIN”. MARÍA LARRINAGA (Department of Cell Biology and Histology, Faculty of Medicine and Nursing, Leioa, Spain)

CHAIR: DR. EDORTA SANTOS (University of the Basque Country (EHU), Vitoria, Spain).

10:00 Sponsor Talk 1 (ONI BIO)

10:15 Sponsor Talk 2 (Everestbiolabs)

CHAIR & MODERATOR: DR. JOAQUIM VIVES (Cell Therapy Service Blood and Tissue Bank, Barcelona, Spain)

11:30 “Introduction EUROPEAN NETWORK OF SoHO ESTABLISHMENTS FOR RAPID AND SUSTAINABLE ACCESS TO ATMPs (BTCs4ATMP)”. DR. JOAQUIM VIVES (Cell Therapy Service Blood and Tissue Bank, Barcelona, Spain).

11:40 “The European ecosystem to foster therapeutics”. DR. SOL RUIZ (Head of Biologics, Biotechnology and Advanced Therapies at the Spanish Medicines Agency (AEMPS). Madrid, Spain).

12:15 “A path to a phase-1 ready EV manufacturing process”. DR. CHANTAL MARTIN (Co-founder and Managing Director, Product Development for Capital BioVentures / Founder and Principal Consultant for CMConsulting Inc. Otawa, Canada).

12:50 Selected talk: “TARGETING THE CREEPING FAT INFLAMMATORY NICHE USING A SUSTAINED EXTRACELLULAR VESICLE DELIVERY PLATFORM IN CROHNS DISEASE”. CAROLINA SERENA (Hospital Universitari de Tarragona Joan XXIII, Institut de Recerca Biomèdica Catalunya Sud (IRB CatSud), Tarragona, Spain)

13:00 Discussion moderated by DR. JOAQUIM VIVES.

CHAIRS: DR. PATRICIA ASPICHUETA (University of the Basque Country (EHU), Biobizkaia Health Research Institute and CIBERehd. Bilbao, Spain), MARC LIEBANA JORDAN (CIC bioGUNE-BRTA, Bilbao, Spain).

14:30 Focus Lecture. “The challenges of Isolation and characterization of Extracellular Vesicles”. JAVIER MUÑOZ PERALTA (Biobizkaia Health Research Institute, Bilbao, Spain).

15:00 Selected talk: Matrix-Specific Binding Assay and Single-Vesicle Resolution Uncovers Distinct Subpopulations of Umbilical Cord MSC-Derived EVs. ANA TALAMILLO (Exosomes Laboratory, CIC bioGUNE-BRTA, CIBERehd, Bizkaia Technology Park, Derio, Bizkaia, Spain)

15:10 Selected talk: HIGH-SENSITIVITY FLOW CYTOMETRY FOR MONITORING EXTRACELLULAR VESICLE DYNAMICS IN PRECLINICAL BREAST CANCER MODELS. MARÍA FIDEL (Clinical Biochemistry, Drug Delivery & Therapy (CB-DDT), Vall d'Hebron Institute of Research (VHIR), Universitat Autònoma de Barcelona (UAB), Barcelona, Spain)

15:20 Selected talk: IMMUNOGENIC RESPONSE OF GOAT-MILK-ISOLATED EXTRACELLULAR VESICLES DECORATED WITH BIOMOLECULAR PROTEIN CORONA  FOR NOVEL NANOTHERANOSTIC APPLICATIONS. IRENE ZABALA (Instituto de Investigación Sanitaria Gregorio Marañón, Unidad de Medicina y Cirugía Experimental, Madrid, Spain)

CHAIR: DR. SANDRA SOUKUP (Achucarro Basque Center for Neuroscience, Ikerbasque Foundation for Science, Bilbao, Spain).

15:30 Talk 3 (Exodus Bio): High-Efficiency Fully Automated Isolation of Exosomes with the EXODUS Systems. YURI D'ALESSANDRA

(EXODUS BIO)

15:45 Talk 4 (HEKAT): NanoSorter: High throughput EVs sorting for fundamental Research and biomedical applications. DR. WILFRID MAZIER 

(PhD Neurosciences, Partnerships and BD, HEKAT)

CHAIR: DR. MARÍA YAÑEZ-MO (Centro de Biología Molecular Severo Ochoa (CBM-SO), Universidad Autónoma de Madrid. Spain).

CHAIR: DR. ROSA HERNANDEZ (University of the Basque Country (EHU). Vitoria, Spain).

9:00 Keynote Lecture. “Extracellular vesicles as vectors for selective delivery of therapeutic nanoparticles to tumors”. PROF. JESUS SANTAMARIA (Institute of Nanoscience and Materials of Aragón (INMA), CSIC–University of Zaragoza. Department of Chemical and Environmental Engineering, University of Zaragoza. Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Zaragoza, Spain).

9:45 Selected talk: POLYMERIC NANOPARTICLE-LOADED EXTRACELLULAR VESICLES AS BIOMIMETIC NUCLEIC ACID VACCINES. JENNIFER FERNÁNDEZ (Grup d’Enginyeria de Materials (GEMAT), Institut Químic de Sarrià (IQS), Universitat Ramon Llull (URL), Barcelona, Spain)

CHAIR: DR. DAVID GIL (Basque Resource for Electron Microscopy (BREM cryo-EM Facility), Instituto Biofisika (EHU-CSIC), Bilbao, Spain).

10:00 Talk 5 (Secretech): Discover hidden biology with single EV proteomics. RUDI SCHLÄFLI 

10:15 Talk 6 (CYTEC BIO): From Detection to Characterization: Cytek Technology for EV Research. 

CYTEC GUEST SPEAKER

CHAIRS: DR. LEYRE URIGÜEN ECHEVARRIA (University of the Basque Country (EHU)/Biomedical Research Centre in Mental Health Network (CIBERSAM). Bilbao, Spain) and DR. ALFREDO RAMOS-MIGUEL (University of the Basque Country (EHU). Bilbao, Spain).

11:15 Selected talk: VESICULAR RPS6 RELEASED BY ASTROCYTES IN AN EXPERIMENTAL MODEL OF AD REGULATES LOCAL TRANSLATION AND ENHANCES SYNAPTIC INTEGRITY IN NEURONES. JIMENA BALERIOLA (Cajal Neuroscience Center)

11:30 Selected talk: CANNABIDIOL ATTENUATES SARS-COV-2–DERIVED EXTRACELLULAR VESICLE–INDUCED NEUROINFLAMMATION IN OLFACTORY NEUROEPITHELIUM–DERIVED CULTURES AND COGNITIVE IMPAIRMENT IN MICE. BRUNO LEMES (Sao Paulo University - Ribeirao Preto Medical School)

11:45 Selected talk: THE AUTOPHAGY PROTEIN ATG18 REGULATES EXOSOME RELEASE AND RESCUES PATHOGENIC DYSFUNCTION ASSOCIATED TO PARKINSON MUTATION IN SYNAPTOJANIN. SANDRA SOUKUP (Achucarro Basque Center for Neuroscience/Ikerbasque)

12:00 Selected talk: EV-ASSOCIATED PROTEINS AS CANDIDATE BIOMARKERS FOR MULTIPLE SCLEROSIS DIAGNOSIS AND STRATIFICATION. ROCIO DEL CARMEN BRAVO (Biogipuzkoa Health Research Institute)

12:15 Selected talk: BRAIN GENE EDITING VIA INTRAVENOUS DELIVERY OF CRISPR/CAS9 RNPS LOADED INTO RVG-EXTRACELLULAR VESICLES. AMAYA CERVERA (Center of Biomedical Research of La Rioja)

CHAIR: DR. UXIA GURRIARAN (CIC bioGUNE-BRTA, Bilbao, Spain).

12:30 Talk 7 (IZON): Towards Robust Workflows for Large-Scale Extracellular Vesicle Isolation and Single-Particle Characterization. JOSHUA KARAM (PhD)

12:45 Talk 8 (HISTOCELL): EV-enriched secretome biologics: from process development to GMP manufacturing. DR. AIORA CENIGAONANDIA 

(PhD Senior Researcher)

CHAIRS: DR. VERONICA TORRANO (University of the Basque Country (EHU), Bilbao, Spain) and ENRIQUE BASTÓN DE LA FUENTE (CNIO, Madrid, Spain).

14:30 Invited Lecture. “Extracellular vesicles as tools for cancer immunotherapy”. PROF. SUSANNE GABRIELSSON (Division of Immunology and Respiratory Medicine, Department of Medicine, Karolinska Institute, Solna, Sweden).

15:10 Selected talk: RISK-STRATIFIED EFFECTS OF PERIPROSTATIC ADIPOSE-DERIVED EXTRACELLULAR VESICLES ON PROSTATE CANCER PROGRESSION. ALAZNE MORENO (Disease Biomarkers and Molecular Mechanisms Group. Institut d'Investigación Biomèdica Catalunya Sud – IRB CatSud. Joan XXIII University Hospital. Universitat Rovira i Virgili. Tarragona, Spain)

15:20 Selected talk: SECRETION MATTERS: EV-MEDIATED WNT7A SHAPES THE TUMOR MICROENVIRONMENT REVEALING A THERAPEUTIC VULNERABILITY IN TNBC. UXIA GURRIARAN (Exosomes Lab, CIC bioGUNE, Basque Research and Technology Alliance (BRTA), Derio, Bizkaia, Spain)

15:30 Selected talk: EXTRACELLULAR VESICLE LDHA AS A MARKER FOR METABOLIC SUBTYPING OF PANCREATIC DUCTAL ADENOCARCINOMA. PILAR ESPIAU (INMA, CSIC-University of Zaragoza & CIBER-BBN, Zaragoza, Spain)

CHAIR: DR. ROCIO BRAVO MIANA (IIS Biogipuzkoa, San Sebastian, Spain).

15:45 Talk 9 (BD) online session

16:00 Talk 10 (BIO-CONNECT and FIBERCELL): Large-Scale Production of Extracellular Vesicles from High-Density 3D Cell Cultures in Hollow Fiber Perfusion Bioreactors” (online session).  JOHN J.S. CADWELL (President and CEO of FiberCell Systems Inc.)

CHAIR: DR. MARIA PARDO (Health Research Institute of Santiago de Compostela (IDIS), Santiago de Compostela, Spain).

CHAIR: DR. HERNANDO DEL PORTILLO (Barcelona Institute for Global Health (ISGlobal). Institut d’Investigació en Ciències de la Salut Germans Trias i Pujol (IGTP). Badalona, Spain).

9:15 Focus Lecture. “Extracellular Vesicles in Human Interactions with Bacteria and Other Pathogens”. DR. JAVIER SOTILLO (Parasitology Reference and Research Laboratory, National Center for Microbiology, Instituto Salud Carlos III, Madrid, Spain).

9:45 Selected talk: EXTRACELLULAR VESICLES AS MORPHOTYPE-DEPENDENT EFFECTORS IN MYCOBACTERIUM ABSCESSUS. GUILLERMO PASCUAL VÁZQUEZ (Universidad Autónoma de Madrid)

9:55 Selected talk: ORGAN-ON-CHIP STUDIES OF EV-MEDIATED CRYPTIC INFECTIONS IN HUMAN MALARIA. JESSICA MOLINA FANKY (ISGlobal)

10:05 Selected talk: CIRCULATING MICROBIOTA-DERIVED EXTRACELLULAR VESICLES REGULATE SYSTEMIC METABOLIC RESPONSES IN OBESITY. CARLOS CASTAÑO (IRBCatSud)

CHAIR: DR. MARTA CLOS-SANSALVADOR (Institut d’Investigació en Ciències de la Salut Germans Trias i Pujol (IGTP). Badalona, Spain).

10:15 Talk 11 (Solmeglas)

10:30 Talk 12 (Hansabiomed)

CHAIR: DR. MARCEL-LA FRANQUESA BARTOLOME. (Germans Trias i Pujol Research Institute (IGTP), Badalona, Spain. President of GEIVEX)

11:15-11-25 Selected talk: PLATELET-DERIVED EXTRACELLULAR VESICLES CARRY SEVERITY-ASSOCIATED MIRNA AND PROTEOMIC SIGNATURES IN RESPIRATORY ALLERGY. ELENA IZQUIERDO (Instituto de Medicina Molecular Aplicada Nemesio Díez (IMMA-ND), Departamento de Ciencias Médicas Básicas, Facultad de Medicina, Universidad San Pablo-CEU, CEU Universities, Boadilla del Monte, Spain)

11:25-11:35 Selected talk: REPROGRAMMING OF CIRCULATING HOST- AND BACTERIA-DERIVED EXTRACELLULAR VESICLES BY DIETARY INTERVENTIONS IMPROVES METABOLIC DYSFUNCTION IN OBESITY. VICTORIA CEPERUELO (DIAMET Research Group, Institut de Recerca Biomèdica Catalunya Sud (IRB CatSud - Formerly Institut d’Investigació Sanitària Pere Virgili (IISPV)), Hospital Universitari Joan XXIII (HJ23), Tarragona, Spain)

11:35-11:45 Selected talk: PROTEIN SUPRAMOLECULAR ASSEMBLY WITHIN EXTRACELLULAR VESICLES DETERMINES THEIR BIOLOGICAL ACTIVITY. JOAQUIN SERAS (Clinical Biochemistry, Drug Delivery and Therapy Group (CB-DDT), Vall d'Hebron Research Institute (VHIR), Vall d'Hebron University Hospital, Barcelona, Spain)

11:45-11:55 Selected talk: Bioactive Lipidomic Signatures and Prophylactic Barrier-Protective Effects of Human Milk Extracellular Vesicles in a Dynamic Gut-on-Chip Model. JULIA KULIGOWSKI (Spanish Network in Maternal, Neonatal, Child and Developmental Health Research (RICORS- SAMID) (RD24/0013/0014), Health Research Institute Hospital La Fe (IIS La Fe), Valencia, Spain)

11:55-12:05 Selected talk: LIPIDOMIC PROFILING OF URINARY EVS UNCOVERS METABOLIC IMPAIRMENT IN ADPKD AND IDENTIFIES DIAGNOSTIC, PROGNOSTIC AND FOLLOW-UP BIOMARKERS. MARÍA PEREIRA (Group of Genetics and Developmental Biology of Renal Diseases, Nephrology Laboratory, University Clinical Hospital of Santiago de Compostela, Spain.)

12:05-12:15 Selected talk: PROTEOMIC AND PHARMACOKINETIC DETERMINANTS OF EXTRACELLULAR VESICLE-MEDIATED ERYTHROPOIETIN DELIVERY IN CHRONIC KIDNEY DISEASE-DERIVED INFLAMMATORY ANEMIA. ZURIÑE ERANA (NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy (EHU). Bioaraba, NanoBioCel Research Group, Vitoria-Gasteiz, Spain)